yt medium Search Results


escherichia coli top10 cells  (Thermo Fisher)
94
Thermo Fisher escherichia coli top10 cells
FIG. 2. Expression and purification of CopA from A. fulgidus. Lanes 1 and 4, 5-g crude membrane fraction of uninduced E. coli transformed with pBAD/TOPO-CopA; lanes 2 and 5, 10-g crude mem- brane fraction of arabinose-induced E. coli transformed with pBAD/ TOPO-CopA; lanes 3 and 6, 5 g of solubilized CopA purified by Ni2- nitrilotriacetic acid column. Lanes 1–3, 10% SDS-PAGE stained with Coomassie Brilliant Blue. Lanes 4–6, blot immunostained with anti-V5 epitope antibody.
Escherichia Coli Top10 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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escherichia coli top10 cells - by Bioz Stars, 2026-09
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yt broth type culture medium  (Thermo Fisher)
95
Thermo Fisher yt broth type culture medium
FIG. 2. Expression and purification of CopA from A. fulgidus. Lanes 1 and 4, 5-g crude membrane fraction of uninduced E. coli transformed with pBAD/TOPO-CopA; lanes 2 and 5, 10-g crude mem- brane fraction of arabinose-induced E. coli transformed with pBAD/ TOPO-CopA; lanes 3 and 6, 5 g of solubilized CopA purified by Ni2- nitrilotriacetic acid column. Lanes 1–3, 10% SDS-PAGE stained with Coomassie Brilliant Blue. Lanes 4–6, blot immunostained with anti-V5 epitope antibody.
Yt Broth Type Culture Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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yt broth type culture medium - by Bioz Stars, 2026-09
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2 x yt medium  (Corning Life Sciences)
90
Corning Life Sciences 2 x yt medium
FIG. 2. Expression and purification of CopA from A. fulgidus. Lanes 1 and 4, 5-g crude membrane fraction of uninduced E. coli transformed with pBAD/TOPO-CopA; lanes 2 and 5, 10-g crude mem- brane fraction of arabinose-induced E. coli transformed with pBAD/ TOPO-CopA; lanes 3 and 6, 5 g of solubilized CopA purified by Ni2- nitrilotriacetic acid column. Lanes 1–3, 10% SDS-PAGE stained with Coomassie Brilliant Blue. Lanes 4–6, blot immunostained with anti-V5 epitope antibody.
2 X Yt Medium, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2 x yt medium - by Bioz Stars, 2026-09
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2× yt medium  (Becton Dickinson)
90
Becton Dickinson 2× yt medium
FIG. 2. Expression and purification of CopA from A. fulgidus. Lanes 1 and 4, 5-g crude membrane fraction of uninduced E. coli transformed with pBAD/TOPO-CopA; lanes 2 and 5, 10-g crude mem- brane fraction of arabinose-induced E. coli transformed with pBAD/ TOPO-CopA; lanes 3 and 6, 5 g of solubilized CopA purified by Ni2- nitrilotriacetic acid column. Lanes 1–3, 10% SDS-PAGE stained with Coomassie Brilliant Blue. Lanes 4–6, blot immunostained with anti-V5 epitope antibody.
2× Yt Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yt+medium/2%C3%97+yt+medium/pmc09769844-222-2-17
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2×yt agar medium  (Cold Spring Harbor Laboratory Press)
90
Cold Spring Harbor Laboratory Press 2×yt agar medium
FIG. 2. Expression and purification of CopA from A. fulgidus. Lanes 1 and 4, 5-g crude membrane fraction of uninduced E. coli transformed with pBAD/TOPO-CopA; lanes 2 and 5, 10-g crude mem- brane fraction of arabinose-induced E. coli transformed with pBAD/ TOPO-CopA; lanes 3 and 6, 5 g of solubilized CopA purified by Ni2- nitrilotriacetic acid column. Lanes 1–3, 10% SDS-PAGE stained with Coomassie Brilliant Blue. Lanes 4–6, blot immunostained with anti-V5 epitope antibody.
2×Yt Agar Medium, supplied by Cold Spring Harbor Laboratory Press, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2-yt medium  (Cold Spring Harbor Laboratory Press)
90
Cold Spring Harbor Laboratory Press 2-yt medium
FIG. 2. Expression and purification of CopA from A. fulgidus. Lanes 1 and 4, 5-g crude membrane fraction of uninduced E. coli transformed with pBAD/TOPO-CopA; lanes 2 and 5, 10-g crude mem- brane fraction of arabinose-induced E. coli transformed with pBAD/ TOPO-CopA; lanes 3 and 6, 5 g of solubilized CopA purified by Ni2- nitrilotriacetic acid column. Lanes 1–3, 10% SDS-PAGE stained with Coomassie Brilliant Blue. Lanes 4–6, blot immunostained with anti-V5 epitope antibody.
2 Yt Medium, supplied by Cold Spring Harbor Laboratory Press, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yt+medium/2+yt+medium/us07892543-802-25-35
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2-yt medium - by Bioz Stars, 2026-09
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2x yt-g medium  (Pharmacia LKB Biotechnology Inc)
90
Pharmacia LKB Biotechnology Inc 2x yt-g medium
FIG. 2. Expression and purification of CopA from A. fulgidus. Lanes 1 and 4, 5-g crude membrane fraction of uninduced E. coli transformed with pBAD/TOPO-CopA; lanes 2 and 5, 10-g crude mem- brane fraction of arabinose-induced E. coli transformed with pBAD/ TOPO-CopA; lanes 3 and 6, 5 g of solubilized CopA purified by Ni2- nitrilotriacetic acid column. Lanes 1–3, 10% SDS-PAGE stained with Coomassie Brilliant Blue. Lanes 4–6, blot immunostained with anti-V5 epitope antibody.
2x Yt G Medium, supplied by Pharmacia LKB Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2x yt-g medium - by Bioz Stars, 2026-09
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2 yt medium  (Becton Dickinson)
90
Becton Dickinson 2 yt medium
FIG. 2. Expression and purification of CopA from A. fulgidus. Lanes 1 and 4, 5-g crude membrane fraction of uninduced E. coli transformed with pBAD/TOPO-CopA; lanes 2 and 5, 10-g crude mem- brane fraction of arabinose-induced E. coli transformed with pBAD/ TOPO-CopA; lanes 3 and 6, 5 g of solubilized CopA purified by Ni2- nitrilotriacetic acid column. Lanes 1–3, 10% SDS-PAGE stained with Coomassie Brilliant Blue. Lanes 4–6, blot immunostained with anti-V5 epitope antibody.
2 Yt Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2×yt medium agar (1.5% agar)  (Corning Life Sciences)
90
Corning Life Sciences 2×yt medium agar (1.5% agar)
FIG. 2. Expression and purification of CopA from A. fulgidus. Lanes 1 and 4, 5-g crude membrane fraction of uninduced E. coli transformed with pBAD/TOPO-CopA; lanes 2 and 5, 10-g crude mem- brane fraction of arabinose-induced E. coli transformed with pBAD/ TOPO-CopA; lanes 3 and 6, 5 g of solubilized CopA purified by Ni2- nitrilotriacetic acid column. Lanes 1–3, 10% SDS-PAGE stained with Coomassie Brilliant Blue. Lanes 4–6, blot immunostained with anti-V5 epitope antibody.
2×Yt Medium Agar (1.5% Agar), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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26 yt medium  (Becton Dickinson)
90
Becton Dickinson 26 yt medium
FIG. 2. Expression and purification of CopA from A. fulgidus. Lanes 1 and 4, 5-g crude membrane fraction of uninduced E. coli transformed with pBAD/TOPO-CopA; lanes 2 and 5, 10-g crude mem- brane fraction of arabinose-induced E. coli transformed with pBAD/ TOPO-CopA; lanes 3 and 6, 5 g of solubilized CopA purified by Ni2- nitrilotriacetic acid column. Lanes 1–3, 10% SDS-PAGE stained with Coomassie Brilliant Blue. Lanes 4–6, blot immunostained with anti-V5 epitope antibody.
26 Yt Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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26 yt medium - by Bioz Stars, 2026-09
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yt medium (2x) premixed powder  (Applichem inc)
90
Applichem inc yt medium (2x) premixed powder
FIG. 2. Expression and purification of CopA from A. fulgidus. Lanes 1 and 4, 5-g crude membrane fraction of uninduced E. coli transformed with pBAD/TOPO-CopA; lanes 2 and 5, 10-g crude mem- brane fraction of arabinose-induced E. coli transformed with pBAD/ TOPO-CopA; lanes 3 and 6, 5 g of solubilized CopA purified by Ni2- nitrilotriacetic acid column. Lanes 1–3, 10% SDS-PAGE stained with Coomassie Brilliant Blue. Lanes 4–6, blot immunostained with anti-V5 epitope antibody.
Yt Medium (2x) Premixed Powder, supplied by Applichem inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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yt medium (2x) premixed powder - by Bioz Stars, 2026-09
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2yt agar becton, dickinson difcotm 2×yt yeast extract tryptone medium  (Becton Dickinson)
90
Becton Dickinson 2yt agar becton, dickinson difcotm 2×yt yeast extract tryptone medium
FIG. 2. Expression and purification of CopA from A. fulgidus. Lanes 1 and 4, 5-g crude membrane fraction of uninduced E. coli transformed with pBAD/TOPO-CopA; lanes 2 and 5, 10-g crude mem- brane fraction of arabinose-induced E. coli transformed with pBAD/ TOPO-CopA; lanes 3 and 6, 5 g of solubilized CopA purified by Ni2- nitrilotriacetic acid column. Lanes 1–3, 10% SDS-PAGE stained with Coomassie Brilliant Blue. Lanes 4–6, blot immunostained with anti-V5 epitope antibody.
2yt Agar Becton, Dickinson Difcotm 2×Yt Yeast Extract Tryptone Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIG. 2. Expression and purification of CopA from A. fulgidus. Lanes 1 and 4, 5-g crude membrane fraction of uninduced E. coli transformed with pBAD/TOPO-CopA; lanes 2 and 5, 10-g crude mem- brane fraction of arabinose-induced E. coli transformed with pBAD/ TOPO-CopA; lanes 3 and 6, 5 g of solubilized CopA purified by Ni2- nitrilotriacetic acid column. Lanes 1–3, 10% SDS-PAGE stained with Coomassie Brilliant Blue. Lanes 4–6, blot immunostained with anti-V5 epitope antibody.

Journal: Journal of Biological Chemistry

Article Title: Characterization of a Thermophilic P-type Ag+/Cu+-ATPase from the ExtremophileArchaeoglobus fulgidus

doi: 10.1074/jbc.m109964200

Figure Lengend Snippet: FIG. 2. Expression and purification of CopA from A. fulgidus. Lanes 1 and 4, 5-g crude membrane fraction of uninduced E. coli transformed with pBAD/TOPO-CopA; lanes 2 and 5, 10-g crude mem- brane fraction of arabinose-induced E. coli transformed with pBAD/ TOPO-CopA; lanes 3 and 6, 5 g of solubilized CopA purified by Ni2- nitrilotriacetic acid column. Lanes 1–3, 10% SDS-PAGE stained with Coomassie Brilliant Blue. Lanes 4–6, blot immunostained with anti-V5 epitope antibody.

Article Snippet: Escherichia coli Top10 cells (Invitrogen, Carlsbad, CA) were transformed with this construct and grown at 37 °C in 2 YT medium supplemented with 150 g/ml ampicillin, and expression was induced with 0.002% L-arabinose.

Techniques: Expressing, Purification, Membrane, Transformation Assay, SDS Page, Staining